Natural food biopreservatives have always’s remained the preference of health consciousconsumers. This necessity has led to the studies exploring better alternativeswhich are more acceptable, economical and safer than chemical preservatives. Bacteriocinsare one of such compounds produced by lactic acid bacteria which offera great potential to contribute in food, health and pharmaceutical industry. Presentreview focuses the complete biochemical, functional and molecular genetic characterizationof bacteriocins produced by Pediococcus spp. A great deal of diverseheterologous expression systems have been exploited for cloning, expression andpurification of pediocins at laboratory scale but data is lacking for industrial processes.Thus, there is an urgent need to design low cost, industrially viable and continuoussystem in order to exploit these natural bioactive compounds in food andpharmaceutical industry