Effects of gamma and electron beam irradiation on viability and DNA elimination of Staphylococcus aureus

Owing to the inability of real-time polymerase chain reaction (PCR)to distinguish between signals originating from viable cells and DNA released fromdead cells, (i.e. it can detect dead cells) a risk exists that DNA from dead cells can resultin false-positive PCR signals, so it is important to use a proper method for sterilization to maintain the accuracy of the quantitative real-time PCR assay.

Author(s): Mona M. K. Shehata, Fatma Alzahraa M. Gomaa, Zeinab H. Helal

Abstract | Full-Text | PDF

Share this