Screening of Lactobacillus isolates for their adherence capabilities to mammalian cells and their acid and bile tolerance

Title: Screening of Lactobacillus isolates for their adherence capabilities to mammalian cells and their acid and bile tolerance. Background: Lactobacilli resemble a major part of the commensal human mucosal flora. The application of Lactobacilli as probiotics has increased during the last few years since a health promoting effect has been reported in addition to their long history of safe use. Methods and finding: In this study, fifty-two Lactobacillus isolates were recovered from dairy products or infant stools were examined in vitro for their probiotic potential. Their adherence capacities to Vero cells in addition to their acid and bile tolerance were evaluated. Only few isolates weren’t able to adhere to Vero cells, while the other isolates have high to moderate adherence capacities. The majority of isolates were tolerant to acid and about 70% were tolerant to 0.3% bile salts. Conclusion: thirty two Lactobacillus isolates were found to possess desirable probiotic properties. These isolates are good candidates for further investigation in in vitro and in vivo studies for their potential health benefits and their application as novel Biotherapeutic agents.

Author(s): Amira Abdel-Daim, Mohamed Seif Aldeen Ashor, Nadia Abdel-Halim Hassouna, Mohamed Mostafa Hafez, Mohammad Mabrouk Aboulwafa

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